Journal of Apicultural Science

Occurrence of Paenibacillus larvae spores in honey samples domestic apiaries

Journal of Apicultural Science. Volume 52, Issue 2, Pages 105-111, ISSN (Print) 1643-4439
The American foulbrood (AFB) Histolysis infectiosa perniciosa larvae apium is an infectious and highly contagious disease of honey bee brood (Apis mellifera) and other Apis species. Recognition of the AFB in apiaries should be confirmed with laboratory tests. These are tests used in detection of the Paenibacillus larvae presence in infected brood. Tests of honey (food stores) for presence of the Paenibacillus larvae can detect the infection in bee colonies before the outbreak of the disease and its clinical symptoms. The aim of the research from the years 2005 and 2007 was to do an introductory assessment of the Paenibacillus larvae presence in apiaries based on tests of honey samples. A total of 242 honey samples were tested. In microbiological tests, cultivation and microscope methods were employed. In 2005, among 142 samples analysed, 34 were positive for Paenibacillus larvae. That was 23% of the total. The same percentage of infected samples was found in 2007 (from 100 tests 23 were positive). Levels of infection varied. The numbers of spores in 1 gram of honey fluctuated from 10 to over 1000.
American foulbrood, Paenibacillus larvae, spores, diagnostics, honey samples
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